WitrynaSimultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis Leshan Xiu,1 Chi Zhang,1 Yamei Li,1 Feng Wang,2 Junping Peng11NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical … WitrynaHerpes simplex viruses (HSV) and varicella-zoster virus (VZV) are members of the herpesviridae family of DNA viruses. A herpes simplex virus infection can cause cutaneous or mucosal lesions that are usually self-limited, but severe disease can occur in immunocompromised patients, pregnant woman and infants. 1,2 HSV-1 is typically …
Detection of varicella zoster virus in genital specimens using a ...
For assay calibration, HHV-6 (U1102 type A strain and Z29 type B strain), HHV-7 (RK strain), HHV-8 (GK strain) standard strains were obtained from the American Type Culture Collection (ATCC; Maryland, USA). These standard strains were then used to obtain recombinant plasmid and test the specificity of the … Zobacz więcej Blood samples from 74 blood donors were collected between August 2024 and December 2024 from the blood donation center in Hubei Provincial Hospital of Traditional Chinese Medicine (Wuhan, Hubei, China). … Zobacz więcej According to previous studies [15, 16], U67, U36, and ORF65 were chosen as the target gene for HHV-6, 7, and 8, respectively. U67 sequences of HHV-6A and HHV-6B, … Zobacz więcej Particular protection measures were taken to avoid sample-to-sample contamination: different rooms and dedicated equipment were used for DNA extraction and processing. Furthermore, for PCR setup and analysis, all … Zobacz więcej The plasmids of HHV-6, 7, and 8 (containing 76 bp U67 amplicon, 147 bp U36 amplicon, and 108 bp ORF65 amplicon, … Zobacz więcej Witryna11 kwi 2008 · The multiplex PCR was designed to qualitatively measure genomic DNA of eight human herpes viruses, that is, herpes simplex virus type 1 (HSV-1), type 2 … phl to palm springs international airport
Evaluation of multiplex real-time polymerase chain reaction ...
WitrynaHerpes simplex viruses types 1 and 2 (HSV-1 and HSV-2), and varicella-zoster virus (VZV) are common agents resulting in various forms of clinical manifestation from skin vesicle to disseminated viral infection. The aim of the present study was to develop a real-time PCR and melting curve analysis which detect and differentiate HSV-1, HSV … WitrynaA polymerase chain reaction (PCR) test may also be performed to determine whether the virus itself is present in the patient's blood. A sample of the patient's blood is taken … WitrynaTaqPath Menu GeneProof MH/UU/UP Multiplex PCR Kit 100 reactions A58123 TaqPath Menu GeneProof Mycoplasma genitalium/hominis PCR Kit 100 reactions A58122 ... TaqPath Menu GeneProof Herpes Simplex Virus (HSV-1/2) PCR Kit 100 reactions A58128 TaqPath Menu GeneProof Universal Positive Control 100 … phl to o\u0027hare