Lysotracker染色后固定
WebDescription. LysoTracker Red DND-99 is a cell-permeable red fluorescent dye that stains acidic compartments within a cell, such as lysosomes. Red DND-99 has an excitation and emission maximum of 577/590 nm and can be efficiently excited using a TRITC filter. LysoTracker probes are available in a variety of fluorescent colors. WebCite. 14th Dec, 2024. Xiliang Du. Jilin University. Our results revealed that 4%paraformaldehyde fixed cell was OK to detect LysoTracker. The protocol was followed: cell was stained with ...
Lysotracker染色后固定
Did you know?
Web25 sept. 2007 · In order to investigate the relationship of Lysotracker Red to the emergent green fluorescence, we compared the mean fluorescent signal obtained by excitation with a 488nm laser or a 594 nm laser ... Web17 iun. 2024 · Summary. We have outlined the approach of visualizing autophagy specifically in the epithelial follicle stem cells of the Drosophila ovary using the LysoTracker dye. The advantage of using this protocol is that it details several techniques, including ovary dissection, immunofluorescence, and western blotting, that positively identify …
WebLysoTracker探针，由荧光基团连接到一个弱碱性基团组成，其中仅弱碱基团可部分提供质子，以维持pH在中性值。 这使LysoTracker探针可自由无阻地穿过细胞膜，特征性的聚集 … Web11 iul. 2013 · LysoTracker dyes do not specifically label lysosomes or autophagosomes within the cell, they allow the simultaneous measurement of an autophagy-related process and other live-cell functions, which are not possible with the standard LC3B antibody-labeling tech-nique. This method has the advantage of other live-cell LCB-GFP-tagged …
Web如果经Lyso-Tracker Red染色后的细胞需要进行固定操作，可以尝试3%的戊二醛 (glutaraldehyde)。 按照1:20000的比例稀释，可以配制1000ml Lyso-Tracker Red工作液。 包装清单： 保存条件： -20℃避光保存，半年有效。 注意事项： Lyso-Tracker Red (1mM)在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内，可以20-25℃水 … WebLyso-Tracker Green适用于活细胞染色，但不适合用于固定后细胞的染色。 如果经Lyso-Tracker Green染色后的细胞需要进行固定操作，可以尝试3%的戊二醛 (glutaraldehyde) …
Web11 oct. 2012 · Use of LysoTracker to detect programmed cell death in embryos and differentiating embryonic stem cells. Programmed cell death (PCD) occurs in adults to …
Web19 aug. 2015 · LysoTracker Green DND-26 is a cell-permeable, non-fixable, green fluorescent dye that stains acidic compartments within a cell, such as lysosomes. Green DND-26 has an excitation and emission maximum of 504/511 nm and can be efficiently excited using a FITC filter. LysoTracker probes are available in a variety of fluorescent … buy diablo 4 cheapWeb可以的。 注意几点: 1.lysotracker和Hochest都可以染活细胞，直接加进培养基里就行，染完用PBS洗掉，即可拍照。 2.一般lysotracker是红色或者绿色通道的荧光，Hochest是蓝色的，可以兼容。 3.不同细胞着色程度有差异，建议调整下染料浓度和染色时间，第一次染时可以在荧光显微镜下每隔10分钟观察一下，或者请教有经验的师兄师姐。 编辑于 2024-12 … buy diablo onlineWebLysoTrackers are cell-permeable lysosome markers that target and track acidic organelles in live cells. LysoTracker markers are comprised of a fluorophore bound to weakly basic … cell phone repair dickerson rdWeb2 sept. 2014 · LysoTracker is an acidotropic dye that stains cellular acidic compartments, including lysosomes and autolysosomes. LysoTracker has been used to detect autophagy-associated lysosomal activity in Drosophila tissues including the fat body, midgut, salivary gland and ovary, as well as in Drosophila cell culture. A low level of LysoTracker … buy diablo 3 charactersWeb16 apr. 2013 · Mitophagy is a specialized form of autophagy that removes damaged mitochondria, thereby maintaining efficient cellular metabolism and reducing cellular stress caused by aberrant oxidative bursts. Deficits in mitophagy underlie several diseases, and a substantial body of research has elucidated key steps in the pathways that lead to and … cell phone repair daytona beach flWeba. 去除细胞培养液，加入步骤 1 配制好的并 37℃预温育的 Lyso-Tracker Red 染色工作液， 与细胞 37℃共孵育 30-120 分钟。 b. 去除 Lyso-Tracker Red 染色工作液，加入新鲜的细胞培养液。 c. 随后通常用荧光显微镜或激光共聚焦显微镜进行观察。 此时可观察到溶酶体呈明亮的强 荧光染色。 如果染色效果欠佳， 可以提高 Lyso-Tracker Red 染色工作液中 Lyso … cell phone repair daytona beach floridaWebLysoTracker™ Red DND-99 是一种红色荧光染料，可用于标记和追踪活细胞中的酸性细胞器。. LysoTracker™ 探针共有的特点包括：. • 对酸性细胞器具有高度选择性. • 醛固定 … buy diablo ii resurrected pc