WebAddgene inc retroviral pbabe puro mcherry egfp lc3b plasmid. Retroviral Pbabe Puro Mcherry Egfp Lc3b Plasmid, supplied by Addgene inc, used in various techniques. Bioz … Webgfp是酸敏感型gfp蛋白，而mrfp是稳定的荧光表达基团，不受外界影响。 由于自噬小体进入第二阶段后，与溶酶体进行融合，形成自噬溶酶体。 自噬溶酶体由于溶酶体内部的酸性 …

pSuperFOXO3s_价格-品牌-详情介绍_丁香通

Web2 jun. 2011 · LC3 is a mammalian homolog of the yeast ATG8 protein, a ubiquitin-like protein that becomes lipidated and tightly associated with the autophagosomal … Webtagged LC3 (tfLC3) reporters such as mRFP-EGFP-LC3 and mCherry-EGFP-LC3 have been developed as single-molecule probes to detect autophagosomes and autolysosomes specifi-cally.6 In green and red merged images with this probe, auto-phagosomes are labeled with yellow (mRFP and GFP) and autolysosomes are labeled with red (mRFP … download shapefile hidrografia ana

LC3双荧光自噬慢病毒的应用（上篇）——实验原理及病毒使用

WebThe whole mCherry-GFP-LC3 expression cassette was excised from a plasmid kindly provided by Terje Johansen's laboratory, and inserted into a shuttle proviral plasmid, containing both the AAV2/2 inverted terminal repeats and the 1.6-kb cytomegalovirus enhancer promoter (Fig. 1 A). Web细胞自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至细胞自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。 当细胞自噬溶酶体形成后，酸性的溶酶体环境使GFP荧光淬灭，而mCherry荧光不受影响，细胞自噬溶酶体呈现红色荧光（图6）。 WebpCMV-mCherry-p62是碧云天研发的在哺乳动物细胞中表达红色mCherry标签的人源p62融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry标签的p62融合蛋白，呈现明亮的红色荧光，可以用于细胞自噬(autophagy)的研究 downloads hannah