P416tef
WebT-Det® LF 416 is a low foaming nonionic surfactant. It is soluble in water at temperatures below its cloud point. It is soluble in polar solvents such as alcohols and glycol ethers and … WebThe only plasmid backbone used for expression of proteins in this part of the lab-work was p416TEF, digested with XbaI and XhoI. The plasmids consecutive promoter, TEF1, as well as terminator, CYC1, were both kept and used for the expression of our protein. The plasmid also contains the bacterial ampR gene, as well as a the yeast URA3 marker gene.
P416tef
Did you know?
WebDec 24, 2024 · Human ATP7B mRNA was cloned in p416TEF vector (as a positive control) and confirmed by sequencing. LmATP7, GFP-LmATP7, and human ATP7B constructs along with empty vector p416TEF were used to transform Wt and ccc2Δ strains. Yeast transformants were selected and maintained on SD medium without uracil (SD-Ura) at 30 … WebPlasmid loss in CEN.PK113-11C carrying an empty plasmid (p416TEF) or an RjCouR/RpCouR expressing plasmid (pDL030/pDL031). Cells were cultured in Delft medium with histidine supplementation overnight.
WebMPY17 cells containing p416TEF (vector) or constitutively expressing Ctr1wt or Ctr1 Met-127 mutant alleles to alanine (M127A), leucine (M127L), cysteine (M127C), serine (M127S), and histidine (M127H) were assayed for growth on YPEG for 5 days at 30 °C and 3 days in SC-ura and YPEG + Cu.D, yeast Ctr1 Met-127 is essential for copper transport. WebMay 1, 2024 · Glutaredoxins are small proteins of the thioredoxin superfamily that are present throughout life. Most glutaredoxins fall into two major subfamilies. Class I glutaredoxins are glutathione-dependent...
WebJan 7, 2024 · Product Name : p416TEF-NYFP article : bacterial resistance : Ampicillin cloning : growth strain : Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+]. … WebConstruction of p416TEF-MFα-prepro Plasmid p416TEF-MFα-prepro (Figure 2.2) was derived from p416-TEF by cloning the MFα-prepro sequence (Kurjan and Herskowitz 1982) into p416-TEF by gap-repair (Orr-Weaver and Szostak 1983). The MFα-prepro sequence was PCR amplified from yeast genomic DNA using primers MFα_F:NheI
WebYeast centromeric expression vector p416TEF Taxonomy ID: 422499 (for references in articles please use NCBI:txid422499) current name. Yeast centromeric expression vector p416TEF. NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 11 (Bacterial, Archaeal and Plant Plastid)
WebWhen low lift is commanded, oil pressure retracts a locking pin within the rocker arm. The rocker arm solenoid valve receives a signal from the ECM. The ECM controls the rocker … ps4 gaming monitor reddit 2019WebJun 3, 2011 · Plasmid p416TEF-CgCYN1 prepared from a dam −dcm− E. coli strain was digested with BclI and a HisG-Ura3-HisG cassette inserted in this site. This cassette was released from plasmid PHUKH2M (derived from pHUKH2 ( 15) and lacks the BamHI and XhoI sites), and the resulting ligation yielded p416TEF-CgCYN1M:HisG-Ura3-HisG. retool dickerson rd nashvilleWebPCR products were spliced by DUG1BamHIF and DUG1HisSalIR primers and cloned at the BamHI-SalI site of p416TEF. E586A mutation was created by using the Stratagene site-directed mutagenesis kit using DUG2E586AF and DUG2E586AR primers. Mutations were confirmed by DNA sequencing. Cloning of M20A Domain of DUG2 retool external usersWebof the constitutive TEF promoter (p416TEF and/or p413TEF vectors) were grown in selective media (SC-ura, or SC-ura-His) to exponential phase (A 600 nm 0.5–1.5). Radioactive 64Cu (specific activity of 15–30 mCi/ g of CuCl 2, Mallinckrodt Institute of Radiology at Washington University, Saint Louis) was added to 1 ml of cell culture to a final ps4 ghostingWebMar 16, 2012 · PCR products were spliced by DUG1BamHIF and DUG1HisSalIR primers and cloned at the BamHI-SalI site of p416TEF. E586A mutation was created by using the Stratagene site-directed mutagenesis kit using DUG2E586AF and DUG2E586AR primers. Mutations were confirmed by DNA sequencing. Cloning of M20A Domain of DUG2 retool downloadWebJun 3, 2011 · Plasmid p416TEF-CgCYN1 prepared from a dam − dcm − E. coli strain was digested with BclI and a HisG-Ura3-HisG cassette inserted in this site. This cassette was … retool font awesomeWebMar 1, 2024 · The p416TEF plasmid was used for plasmid construction and as the control. Δfat1 transformed with p416TEF was able to grow in the presence of α-linolenic acid, whereas the growth of Δfat1 transformed with p416TEF–CmFAX1 was significantly inhibited in a manner similar to the S. cerevisiae wild-type (Fig. 2 A, left). retooled game