Strand exchange amplification
WebRecently, a strand exchange amplification (SEA), mediated by denaturation bubbles, was established by Shi et al. (2016). Similarly to the traditional PCR technique, SEA employed a … Web10 Jul 2024 · A novel detection method named accelerated strand exchange amplification is reported, achieving direct detection of 120 copies of DNA within 15 min and 1.2 × 105 copies of RNA within 20 min and sparking the revolution of the use of routine isothermal polymerases for diverse applications. We reported a novel detection method named …
Strand exchange amplification
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Web15 Mar 2024 · A strand exchange amplification (SEA) method to detect foodborne pathogen Listeria monocytogenes was developed. SEA is a novel nucleic acid amplification method that only requires one pair of primers. The specie-specific primers were designed by targeting the 16S rRNA gene and the amplification reaction was performed as short as 60 … Web29 Jan 2024 · Denaturation Bubble-mediated Strand Exchange Amplification (SEA) was established, which is an isothermal method with only a primer pair and one Bst DNA …
WebHerein, a simple, rapid, and effective method, accelerated strand exchange amplification (ASEA), was developed for EBV detection, which could offer a strategy for non-invasive … Web19 Nov 2024 · DNA strand displacement amplification is carried out under isothermal conditions and therefore does not need expensive instruments. It is simple, fast, sensitive, …
Web6 Sep 2016 · Here, we introduced the concept of strand exchange amplification (SEA) mediated by denaturation bubbles. Similar to traditional PCR, it only employed a DNA … Web7 Apr 2024 · The MarketWatch News Department was not involved in the creation of this content. Apr 07, 2024 (The Expresswire) -- The purpose of this report onIsothermal Nucleic Acid Amplification Technology ...
Web14 Sep 2024 · With good amplification efficiency for short-sequence targets, ESEA is an ideal choice for the point-of-care testing of SARS-CoV-2 with a high mutation rate. ESEA reaction can be completed in one step and verified by restriction enzyme digestion. The design consisting of three working primers greatly improved the amplification efficiency.
61文章Web14 Sep 2024 · With good amplification efficiency for short-sequence targets, ESEA is an ideal choice for the point-of-care testing of SARS-CoV-2 with a high mutation rate. ESEA … 61敗者WebThus, we developed accelerated denaturation bubble-mediated strand exchange amplification (ASEA) for CPV detection, and it is a rapid, convenient, and cost-effective method. ASEA was able to distinguish CPV genomic DNA in a mixture that included canine genomic DNA as well as nucleic acids sourced from nine other common pathogens, ... 61文件是什么格式Web3 Feb 2024 · H. pylori can be isolated from human saliva, and the sampling of saliva is easy and convenient. Therefore, we developed a visual denaturation bubble-mediated strand … 61按键Web熊二虎，“潇湘学者”特聘教授，硕士生导师，湖南省青年科技人才-荷尖人才（2024年）。. 2024年10月博士毕业于湖南大学化学专业（导师：陈金华教授），2016年9月-2024年9月国家公派到美国德克萨斯大学奥斯汀分校进行博士联合培养（导师：Andrew D. Ellington教授 ... 61教育Web21 Feb 2024 · INTRODUCTION. Homologous recombination (HR) is a DNA strands exchange process essential for multiple pathways of genome maintenance and plasticity in all kingdoms of life (1, 2).Defects in any of these pathways lead to deleterious consequences, such as cell death or various types of cancer ().HR relies on the pairing of a single … 61文案Web15 Mar 2024 · Abstract Denaturation bubble-mediated strand exchange amplification (SEA) is a novel, rapid isothermal nucleic acid amplification has been applied for point-of-care … 61方案